CircINSR Regulates Fetal Bovine Muscle and Fat Development

The level of muscle development in livestock directly affects the production efficiency livestock, and contents intramuscular fat (IMF) is an important factor that meat quality. However, molecular mechanisms through which circular RNA (circRNA) IMF remains largely unknown. In this study, we isolated myoblasts preadipocytes from fetal bovine skeletal muscle. Oil Red O BODIPY staining were used to identify lipid droplets preadipocytes, anti-myosin heavy chain (MyHC) immunofluorescence was myotubes differentiated myoblasts. Bioinformatics, a dual-fluorescence reporter system, pull-down, RNA-binding protein immunoprecipitation determine interactions between circINSR micro (miR)-15/16 family. Molecular biochemical assays confirm roles played by preadipocytes. We found able differentiate normally. CircINSR serve as sponge for miR-15/16 family, targets CCND1 Bcl-2 . overexpression significantly promoted myoblast preadipocyte proliferation inhibited cell apoptosis. addition, adipogenesis alleviating inhibition against target genes FOXO1 EPT1 Taken together, our study demonstrated serves regulator embryonic development.